Table of Contents

PCR Fundamentals.- PCR Basics.- PCR Methodology.- Laboratory Accreditation Standards.- Clinical Aspects and Diagnosis of Infectious Disease.- Meningitis and Encephalitis.- Respiratory Infections.- Blood-Borne Disease.- Enteric Infections.- Sexually Transmitted Infections.- PCR Protocols for Bacterial Pathogens.- Bordetella pertussis and Bordetella parapertussis.- Brucellosis – The Role of PCR in Diagnosis and Management.- Burkholderia cepacia Complex and Burkholderia gladioli.- Chlamydia and Lymphogranuloma Venereum (LGV).- Chlamydophila.- Coxiella burnetii.- Diarrheagenic Escherichia coli Pathotypes (DEP) Including Enterohaemorrhagic (EHEC)/Shiga-toxin E. coli (STEC).- Haemophilus ducreyi and Klebsiella granulomatis.- Haemophilus influenzae – Capsulated and Non-typeable.- Mycobacterium tuberculosis Complex.- Mycobacteria Other Than Mycobacterium tuberculosis.- Mycoplasma genitalium.- Mycoplasma pneumoniae.- Neisseria gonorrhoeae.- Neisseria meningitidis.- Pseudomonas aeruginosa.- Rickettsia.- Streptococcus pneumoniae.- Treponema pallidum.- Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene.- PCR Protocols for Viral Pathogens.- Adenovirus.- Cytomegalovirus (CMV).- Dengue Virus.- Enterovirus.- Epstein Barr Virus.- Flavivirus.- Hepatitis A Virus.- Hepatitis B Virus.- Hepatitis C Virus.- Hepatitis D Virus.- Hepatitis E Virus.- Herpes Simplex Virus Type 1 and 2.- Human Bocavirus.- Human Coronaviruses.- Human Herpes Viruses -6, -7 and -8.- Human Papillomavirus.- Human Polyomaviruses – JCV and BKV.- Human Polyomaviruses – KIV and WUV.- Human Rhinoviruses.- Influenza Virus A H5N1 (“Avian Influenza”).- Influenza Virus A H1N1 (2009) (“Human Swine Influenza”).- Influenza Type C.- Measles Virus.- Norovirus.- Respiratory Syncytial VirusTypes A and B.- Rotavirus.- Varicella Zoster virus.- Eight Commonly Recognised Respiratory Viruses.- Detection of CMV, HSV, VZV, EBV and Enterovirus by Multiplex PCR.- PCR Protocols for Fungal and Parasitic Pathogens.- Aspergillus Species.- Cryptosporidium.- Cyclospora.- Dientamoeba fragilis.- Entamoeba histolytica.- Giardia.- Malaria (P. falciparum, P. vivax, P. malariae, P. ovale).- Microsporidia.- Pneumocystis jirovecii.- Trichomonas vaginalis.- Toxoplasma gondii.- Multiplex PCR for Protozoan Parasites.- Universal Detection and Identification of Fungi by PCR and DNA Sequencing.- Susceptibility Screening.- An Introduction to Antimicrobial Susceptibility Screening.- PCR Assays in Detecting Methicillin Resistance in Staphylococci: Coagulase Negative Staphylococci (CNS), S. aureus, and MRSA with the PVL Gene.- Detection of VRE: vanA and vanB Genes by PCR.- Metallo ? Lactamases Gene blaimp, blaspm and blavim Detection by Multiplex Real-Time TaqMan Assay on the Smartcycler.- PCR-Sequencing for Detection of Human Cytomegalovirus Mutations Conferring Antiviral Resistance.- Erratum to: Mycobacterium tuberculosis Complex.