Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition
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Manipulating the Mouse Embryo

A Laboratory Manual, Fourth Edition

By Richard Behringer, Marina Gertsenstein, Kristina Nagy, Andras Nagy

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Format: Paperback, 814 pages, Revised Edition
Other Information: Illustrated
Published In: United States, 01 October 2013
This 4th edition of the "Mouse Manual"-Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have reorganized and updated this edition to include new information and protocols on:

. assisted reproduction techniques for sperm and embryo cryopreservation
. generation of induced pluripotent stem cells
. isolation, generation, and transplantation of spermatogonial stem cell lines
. in utero electroporation of gene constructs into post-implantation embryos
. vibratome sectioning of live and fixed tissues for imaging thick tissue sections
. whole-mount fluorescent staining methods for three-dimensional visualization.
Techniques regarding recombinant DNA technology and mouse embryonic development from the previous editions have been updated and recast, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. Mouse embryo manipulation techniques have developed exponentially since the first edition, but then, as now, Manipulating the Mouse Embryo remains the essential practical and theoretical guide for anyone working with mice-students, lab technicians, and investigators.


"The editors...should be praised once again for a brilliant work done, accommodating the latest techniques in mouse embryo manipulation, while not forgetting the traditional procedures that must be learnt properly by anyone joining this field of animal transgenesis for the first time." ---International Society for Transgenic Technologies Blog

EAN: 9781936113019
ISBN: 1936113015
Publisher: Cold Spring Harbor Laboratory Press
Dimensions: 27.43 x 21.59 x 4.06 centimeters (2.27 kg)
Age Range: 15+ years
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