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Manipulating the Mouse Embryo

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Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryoperservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.
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Table of Contents

Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future Chapter 2. Summary of Mouse Development Chapter 3. Production of Transgenic and Chimeric Mice: General Issues Chapter 4. Recovery and In Vitro Culture of Pre-implantation-Stage Embryos Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos Chapter 6. Surgical Procedures Chapter 7. Production of Transgenic Mice Chapter 8. Isolation, Culture, and Manipulation of Blastocyst-derived Stem Cells Chapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells Chapter 11. Production of Chimeras Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences Chapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning Chapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection Chapter 15. Cryopreservation, Rederivation, and Transport of Mice Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems Chapter 17. Setting up and Equipping a Micromanipulation Lab Appendices


This manual will become rapidly a reference and find its place in most laboratories. It is usually difficult to reach broad consensus within the scientific field. However, I am sure that the current edition of this classical manual will delightfully and equally satisfy research scientists and specialized technicians within the field of mouse embriology and transgenesis. Undoubtedly, at present, this manual should be the first recommendation to any person willing to work with transgenic or knockout mice. Transgenic Research This manual is a welcome update, which grew out of teaching material for two practical courses in the Molecular Embryology of the Mouse held at Cold Spring Harbor Laboratory in 1983 and 1984. The previous editions of what is affectionately known as the Mouse Manual became and remain the definitive reference sources on this subject. The current authors have taken the mantle of authorship from Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy and have extensively reorganized and rewritten the new edition. It contains 267 more pages than the second edition, which is largely reflective of the significant advances that have occurred since 1994. This is not just a how-to manual for those who seek to manipulate the mouse embryo and genome, but a useful, concise reference on mouse laboratory strain origins, genetics, and embryology and their places in contemporary biology and medicine. The Quarterly Review of Biology

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