Table of Contents

Preface
Acknowledgements
List of Contributors
Abbreviations
1: Norah Spears, Evelyn E. Telfer and Alison A. Murray: Follicle development in vitro
2: Michelle Lane and David Gardner: Preparation of gametes, in vitro maturation, in vitro fertilization and embryo recovery
3: David Gardner and Michelle Lane: Culture of the mammalian preimplantation embryo
4: Denny Sakkas, Michelle Lane and David Gardner: Assessment of embryo development and viability
5: Gianpiero Palermo, Queenie V. Neri, Takumi Takeuchi, Lucinda L. Veeck, Zev Rosenwaks: Micromanipulation : ICSI
6: Joyce Harper and Alpesh Doshi: Micromanipulation : Biopsy
7: Michelle Lane, Jay Baltz and David Gardner: Analysis of intracellular ions: pH and calcium
8: David Gardner and Michelle Lane: Nutrient uptake and metabolite production and enzyme activity and regulation
9: Don Rieger: Metabolic Pathway
10: Calvin Simerly, Ricardo Moreno, João Ramalho-Santos, Laura Hewitson, and Gerald Schatten: Confocal imaging of structural molecules in mammalian gametes
11: Leeanda Wilton: Fluorescent in situ hybridization
12: W. A. King, D. H. Betts, W. Slimane and P.K. Basur: Assessment of ploidy, telomere length and telomerase activity in oocytes and embryos
13: C. Wrenzycki, A.J. Watson, and M. Calder: Microscale RNA isolation from mammalian embryos
14: P. A. De Sousa and A. J. Watson: Relative mRNA transcript abundance in early embryos by Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
15: Dave Natale: Characterization of novel genes during early development by application of differential display RT-PCR
16: K. E. Latham, G. Nourjanova, K. Wigglesworth and J. J. Eppig: Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells
17: Lisa C Barcroft, D Holstead Jones-Taggart and Andrew J Watson: Antisense disruption of gene expression in the preimplantation embryo
18: M. A. Sirard and C. Robert: Gene subtraction and analysis
19: Daniel Brison Anthony D Metcalfe, Debra J Bloor, Helen R Hunter, Gerard Brady, Susan J Kimber: Analysis of apoptosis in the preimplantation embryo
20: D. P. Baluch, C. M. Pauken, and D. G. Capco: Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo
21: Susan E. Lazendorf, Catherine A. Boyd and Diane L. Wright: Human Embryonic Stem
22: Makoto Nagano: Spermatogonial Transplantion
23: Paul De Sousa, William Ritchie, Andras Dinnyes, Tim King, Lesley Paterson, and Ian Wilmut: Somatic Cell Nuclear Transfer
24: Tetsunori Mukaida and Magosaburo Kasai: Cryobiology; slow freezing vitrification of embryos